Detection Of Presence Of Dissimilar Lipid Components Inward An Fossil Oil Sample Past Times Tlc

Principle.

Thin-layer chromatography (TLC) is a technique of chromatography for the separation of a mixture past times using a thin layer of stationary stage supported on the insert plate.

Chemical Required.

Benzene-Methanol, Alcohol, Silica Gel (Chromatographic grade)

Apparatus.

Beakers, Pipette, Slides, Chromatographic tank, Silica Gel TLC Plates, iodine Chamber (place iodine crystals gas jolt together with cover)

Procedure.

1. Take a silica gel coated drinking glass plate.
2. Draw baseline most two cm inward a higher house the base of operations of the plate amongst sparse pencil.
3. Placemarks of given lipid sample past times using a capillary subway scheme on the baseline.
4. Place the plate inward a chromatographic tank containing benzene-methanol (80:20) mixture used equally the solvent.
5. Wait for 45 minutes to 1 lx minutes for the solvent to ascension together with to carve upwards dissimilar fractions of lipid.
6. Remove the plate together with house inward the gas jolt containing iodine vapours produced past times the sublimation of iodine from Its crystals for three to five minutes.
7. Spots of dissimilar colors of the components of lipids volition hold out seen on the plate.

Result.

The stone oil components are separated.

and tin hold out seen on TLC plate.